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SPHK1 antibody

Cat.#: 115565

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Product Information

  • Product Name
    SPHK1 antibody
  • Documents
  • Description
    SPHK1 Rabbit Polyclonal antibody. Positive WB detected in L02 cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 42 kDa
  • Tested applications
    ELISA, IHC, WB
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    SK 1 antibody; sphingosine kinase 1 antibody; SPHK antibody; SPHK1 antibody; SPHK1 phopho antibody; SPK antibody; SPK 1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of SPHK1 recombinant protein (Accession Number: NM_001355139). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:1000

    IHC: 1:20-1:200

  • Validations

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:115565(SPHK1 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:115565(SPHK1 antibody) at dilution of 1:100 (under 10x lens)

    L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:115565(SPHK1 Antibody) at dilution of 1:300

    L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:115565(SPHK1 Antibody) at dilution of 1:300

  • Background
    SPHK(Sphingosine kinase 1) is also named as SPHK, SPK.It catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions and is precariously perched at the balance point between progrowth and pro-death signalling in the cell.Endogenous SPHK1 purified from placenta has identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties(PMID:10947957).It has 2 isoforms produced by alternative splicing and can form the oligomeric structure(PMID:22335554).
  • References
    • Lu ZP, Xiao ZL, Yang Z. Hepatitis B virus X protein promotes human hepatoma cell growth via upregulation of transcription factor AP2α and sphingosine kinase 1. Acta pharmacologica Sinica. 36(10):1228-36. 2015.
    • Lu Z, Zhang W, Gao S. MiR-506 suppresses liver cancer angiogenesis through targeting sphingosine kinase 1 (SPHK1) mRNA. Biochemical and biophysical research communications. 468(1-2):8-13. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"