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Product Name
SPAG6 antibody
- Documents
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Description
SPAG6 Rabbit Polyclonal antibody. Positive WB detected in mouse testis tissue, Jurkat cells, PC-3 cells. Positive IP detected in mouse testis tissue. Observed molecular weight by Western-blot: 50-55 kDa
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Tested applications
ELISA, IP, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp434I153 antibody; pf16 antibody; Protein PF16 homolog antibody; Repro SA 1 antibody; SPAG6 antibody; sperm assOCiated antigen 6 antibody; Sperm flagellar protein antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SPAG6 recombinant protein (Accession Number: NM_172242). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:115534(SPAG6 Antibody) at dilution of 1:300
IP Result of anti-SPAG6 (IP:Catalog No:115534, 3ug; Detection:Catalog No:115534 1:300) with mouse testis tissue lysate 4000ug.
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Background
Sperm-associated antigen 6 (SPAG6) is an axonemal protein ubiquitously expressed in tissues containing ciliated cells. SPAG6 is essential for sperm motility and fertility. As a novel cancer-testis antigen, SPAG6 is overexpressed in myeloid malignancies. Recently, SPAG6 has been found to negatively regulate neuronal migration during mouse brain development.
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References
- Yan R, Hu X, Zhang Q. Spag6 Negatively Regulates Neuronal Migration During Mouse Brain Development. Journal of molecular neuroscience : MN. 57(4):463-9. 2015.
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