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Product Name
SPAG4L antibody
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Description
SPAG4L Rabbit Polyclonal antibody. Positive WB detected in human testis tissue, HeLa cells. Observed molecular weight by Western-blot: 43 kDa
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Tested applications
ELISA, WB
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
dJ726C3.1 antibody; SPAG4L antibody; SUN5 antibody; TSARG4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SPAG4L recombinant protein (Accession Number: NM_080675). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
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Validations
human testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:115531(SPAG4L antibody) at dilution of 1:400
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Background
SPAG4L,also named as SUN domain-containing protein 5 or Testis and spermatogenesis-related gene 4 protein, is a 379 amino acid protein, which contains 1 SUN domain. SPAG4L is widely expressed and localizes in the nucleus inner membrane. SPAG4L is restricted to the apical nuclear region of round spermatids that face the acrosomic vesicle. SPAG4L is involved in linkage of the acrosomic vesicle to the spermatid nucleus, and in acrosome biogenesis.
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References
- Jiang XZ, Yang MG, Huang LH, Li CQ, Xing XW. SPAG4L, a novel nuclear envelope protein involved in the meiotic stage of spermatogenesis. DNA and cell biology. 30(11):875-82. 2011.
- Yassine S, Escoffier J, Abi Nahed R. Dynamics of Sun5 localization during spermatogenesis in wild type and Dpy19l2 knock-out mice indicates that Sun5 is not involved in acrosome attachment to the nuclear envelope. PloS one. 10(3):e0118698. 2015.
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