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Product Name
SPAG4-Specific antibody
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Description
SPAG4-Specific Rabbit Polyclonal antibody. Positive IHC detected in mouse testis tissue. Positive IF detected in HepG2 cells. Positive WB detected in human testis tissue, HepG2 cells. Observed molecular weight by Western-blot: 55 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
SPAG4 antibody; sperm assOCiated antigen 4 antibody; SUN4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_003116). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:115532(SPAG4-Specific Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded mouse testis tissue slide using Catalog No:115532(SPAG4-Specific Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded mouse testis tissue slide using Catalog No:115532(SPAG4-Specific Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:115532(SPAG4-Specific Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SPAG4 may assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail. This antibody is specific to SPAG4.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"