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Product Name
SPACA3 antibody
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Description
SPACA3 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, mouse testis tissue. Positive IF detected in HEK-293 cells. Observed molecular weight by Western-blot: 28-32 kDa,46-50 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
1700025M08Rik antibody; ALLP17 antibody; Cancer/testis antigen 54 antibody; CT54 antibody; LYC3 antibody; Lysozyme like protein 3 antibody; LYZL3 antibody; SLLP1 antibody; SPACA3 antibody; sperm acrosome assOCiated 3 antibody; Sperm lysozyme like protein 1 antibody; SPRASA antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SPACA3 recombinant protein (Accession Number: NM_001317225). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115528(SPACA3 antibody) at dilution of 1:300
Immunofluorescent analysis of HEK-293 cells using Catalog No:115528(SPACA3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SPACA3, also named as LYC3, LYZL3, SLLP1, SPRASA, CT54 and ALLP-17, belongs to the glycosyl hydrolase 22 family. It is a sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane. SPACA3 has a Dimer form(PMID:15982649 ).
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