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Product Name
SPA17 antibody
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Description
SPA17 Rabbit Polyclonal antibody. Positive IP detected in mouse testis tissue. Positive WB detected in mouse testis tissue. Positive IHC detected in human testis tissue, human ovary tumor tissue. Observed molecular weight by Western-blot: 25kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Cancer/testis antigen 22 antibody; CT22 antibody; SP17 antibody; SP17 1 antibody; SPA17 antibody; sperm autoantigenic protein 17 antibody; Sperm protein 17 antibody; Sperm surface protein Sp17 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SPA17 recombinant protein (Accession Number: XM_011542870). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:115526(SPA17 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human testis using Catalog No:115526(SPA17 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:115526(SPA17 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-SPA17 (IP:Catalog No:115526, 3ug; Detection:Catalog No:115526 1:400) with mouse testis tissue lysate 4800ug.
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Background
Sperm surface protein Sp17（SPA17）is a 151 amino acids protein (17kDa), which is a sperm surface peripheral membrane protein. It is a highly conserved mammalian protein expressed in developing spermatozoa and mature spermatids, and reported to play a role in the interaction of sperm with the zona pellucida. SP17 exists as a homodimer and localizes to the head and tail of spermatozoa. SP17 has been identified as a cancer/testis antigen and is expressed in ovarian cancer and multiple myeloma. This suggests that SP17 could be suitable as a target in tumor immunotherapy.
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References
- Sideras K, Bots SJ, Biermann K. Tumour antigen expression in hepatocellular carcinoma in a low-endemic western area. British journal of cancer. 112(12):1911-20. 2015.
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