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Product Name
SNRPA1 antibody
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Description
SNRPA1 Rabbit Polyclonal antibody. Positive IHC detected in human lung tissue, human brain tissue, human kidney tissue, human liver tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue. Positive IF detected in HepG2 cells. Positive IP detected in HepG2 cells. Positive WB detected in HeLa cells, HepG2 cells, Jurkat cells, MCF7 cells, Raji cells. Observed molecular weight by Western-blot: 28 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
SNRPA1 antibody; U2 snRNP A antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SNRPA1 recombinant protein (Accession Number: NM_003090). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115457(SNRPA1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human lung using Catalog No:115457(SNRPA1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung using Catalog No:115457(SNRPA1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using SNRPA1 antibody Catalog No:115457 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-SNRPA1 (IP:Catalog No:115457, 4ug; Detection:Catalog No:115457 1:500) with HepG2 cells lysate 1000ug.
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Background
Small nuclear ribonucleoprotein polypeptide a-prime(SNRPA1) is part of the U2 snRNP particle, which associated with the branch point of the lariat-shaped intron, in intermediate in the cascade of mRNA precursor splicing event. It may be required for cell division, and helps the A' protein to bind stem loop IV of U2 snRNA. This is a rabbit polyclonal antibody raised against the full length SNRPA1 of human origin.
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References
- Kfir N, Lev-Maor G, Glaich O. SF3B1 association with chromatin determines splicing outcomes. Cell reports. 11(4):618-29. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"