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Product Name
SMMHC antibody
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Description
SMMHC Rabbit Polyclonal antibody. Positive IHC detected in human colon tissue, human bladder tissue, human breast hyperplasia tissue, human heart tissue, human liver cancer tissue, human skeletal muscle tissue, human tonsil tissue, rat bladder tissue, rat colon tissue. Positive WB detected in mouse uterus tissue, mouse colon tissue. Observed molecular weight by Western-blot: 200 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
MYH11 antibody; Myosin 11 antibody; Myosin heavy chain 11 antibody; SMHC antibody; SMMHC antibody; SMM-HC antibody; smooth muscle myosin heavy chain antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SMMHC recombinant protein (Accession Number: NM_022844). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
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Validations
mouse uterus tissue were subjected to SDS PAGE followed by western blot with Catalog No:115391(MYH11 antibody) at dilution of 1:3000
Immunohistochemical of paraffin-embedded human skeletal muscle using Catalog No:115391(MYH11 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human skeletal muscle using Catalog No:115391(MYH11 antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human colon tissue slide using Catalog No:115391(MYH11 Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human colon tissue slide using Catalog No:115391(MYH11 Antibody) at dilution of 1:200 (under 40x lens).
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Background
SMMHC (smooth muscle myosin heavy chain; MYH11) is a contractile protein of smooth muscle cells. It is specifically expressed in cells derived from smooth muscle lineages. SMMHC is used as vascular smooth muscle cell (vSMC) contractile marker. It is also an excellent marker for myoepithelial cells, with no or few cross-reaction with myofibroblasts, thus very useful in the evaluation of stromal invasion.
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