SMAD4 antibody

Cat.#: 115419

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Product Information

  • Product Name
    SMAD4 antibody
  • Documents
  • Description
    SMAD4 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells. Positive IHC detected in human placenta. Observed molecular weight by Western-blot: 58kd
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    human, mouse, rat; other species not tested.
  • Alternative names
    SMAD4 antibody; JIP antibody; SMAD family member 4 antibody; Mothers against DPP homolog 4 antibody; DPC4 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Peptide (Accession Number: NM_001407042). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115419(SMAD4 antibody) at dilution of 1:300

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115419(SMAD4 antibody) at dilution of 1:300

    Immunohistochemical of paraffin-embedded human placenta using Catalog No:115419(SMAD4 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human placenta using Catalog No:115419(SMAD4 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human placenta using Catalog No:115419(SMAD4 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human placenta using Catalog No:115419(SMAD4 antibody) at dilution of 1:50 (under 40x lens)

  • Background
    Mammalian homologs of the Drosophila Mad gene include Smad1, Smad2, Smad3, Smad4 (DPC4), Smad5, Smad6, Smad7 and Smad8. Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interactionwith various Smad family members . Smad6 and Smad7 regulate the response to activin/ TGF signaling by interfering with TGF -mediated phosphorylation of other Smad family members. This antibody is a rabbit polyclonal antibody raised against a peptide (25-43aa) mapping within human SMAD4. It is specific to SMAD4.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"