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Product Name
SLC25A1 antibody
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Description
SLC25A1 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, HepG2 cells, rat liver tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human kidney tissue, human breast cancer tissue. Observed molecular weight by Western-blot: 34 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CIC antibody; Citrate transport protein antibody; CTP antibody; SLC20A3 antibody; SLC25A1 antibody; Tricarboxylate carrier protein antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SLC25A1 recombinant protein (Accession Number: NM_005984). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115320(SLC25A1 antibody) at dilution of 1:400
Immunofluorescent analysis of HepG2 cells using Catalog No:115320 (SLC25A1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:115320(SLC25A1 antibody) at dilution of 1:100 (under 40x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:115320(SLC25A1 antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-SLC25A1 (IP:Catalog No:115320, 2ug; Detection:Catalog No:115320 1:500) with HeLa cells lysate 2000ug.
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Background
SLC25A1, also known as CIC or CTP, is a mitochondrial citrate transporter that exports citrate from the mitochondria to the cytosol. SLC25A1 is highly expressed in several tumor types. Recently it has been identified as a novel transcriptional target of mutant p53 and a negative tumor prognostic marker. In addition, defects in SLC25A1 has been found as a cause of combined D-2- and L-2-hydroxyglutaric aciduria. This antibody specifically recognized the endogenous SLC25A1; no protein was detected in cells from subjects containing two alleles with truncating mutations with this antibody. (24681808, 23561848)
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References
- Nota B, Struys EA, Pop A. Deficiency in SLC25A1, encoding the mitochondrial citrate carrier, causes combined D-2- and L-2-hydroxyglutaric aciduria. American journal of human genetics. 92(4):627-31. 2013.
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