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Product Name
SKIV2L antibody
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Description
SKIV2L Rabbit Polyclonal antibody. Positive IP detected in HepG2 cells. Positive WB detected in HEK-293 cells, COLO 320 cells, HepG2 cells, human kidney tissue, Jurkat cells. Observed molecular weight by Western-blot: 138kd
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
170A antibody; DDX13 antibody; Helicase like protein antibody; Helicase SKI2W antibody; HLP antibody; SKI2 antibody; SKI2W antibody; SKIV2 antibody; SKIV2L antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SKIV2L recombinant protein (Accession Number: NM_006929). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115258(SKIV2L Antibody) at dilution of 1:600
IP Result of anti-SKIV2L (IP:Catalog No:115258, 4ug; Detection:Catalog No:115258 1:600) with HepG2 cells lysate 3200ug.
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Background
The Ski complex is a multiprotein complex required for exosome-mediated RNA surveillance, including the regulation of normal mRNA and the decay of nonfunctional mRNA. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C) [PMID:22444670]. SKIV2L , one component of the Ski complex, acts as an RNA helicase with a role in exosome recruitment or activation. It is involved in the degradation of RNAs by the exosome and may have a role in autophagy [PMID:11719186].
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References
- Saito S, Hosoda N, Hoshino S. The Hbs1-Dom34 protein complex functions in non-stop mRNA decay in mammalian cells. The Journal of biological chemistry. 288(24):17832-43. 2013.
- Eckard SC, Rice GI, Fabre A. The SKIV2L RNA exosome limits activation of the RIG-I-like receptors. Nature immunology. 15(9):839-45. 2014.
- Lu F, Shi Y, Qu C. A genetic variant in the SKIV2L gene is significantly associated with age-related macular degeneration in a Han Chinese population. Investigative ophthalmology & visual science. 54(4):2911-7. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"