Product Name

SHP2 antibody

Documents

Description

SHP2 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in Jurkat cells, A431 cells, HEK-293 cells, human brain tissue, human heart tissue, human skeletal muscle tissue, MCF7 cells, NIH/3T3 cells, U-937 cells. Positive IHC detected in human breast cancer tissue, human heart tissue. Positive IF detected in Hela cells, MCF-7 cells. Observed molecular weight by Western-blot: 68-75kd

Tested applications

ELISA, WB, IHC, IF, IP

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

BPTP3 antibody; CFC antibody; NS1 antibody; PTP 1D antibody; PTP 2C antibody; PTP2C antibody; PTPN11 antibody; SH PTP2 antibody; SH PTP3 antibody; SHP 2 antibody; SHP2 antibody; SHPTP2 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of SHP2 recombinant protein (Accession Number: NM_080601). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:500-1:5000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:115280(PTPN11 antibody) at dilution of 1:500

Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:115280(PTPN11 antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:115280(PTPN11 antibody) at dilution of 1:100 (under 40x lens)

Immunofluorescent analysis of Hela cells, using PTPN11 antibody Catalog No:115280 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

IP Result of anti-PTPN11 (IP:Catalog No:115280, 4ug; Detection:Catalog No:115280 1:1000) with HEK-293 cells lysate 2800ug.

Background

PTPN11(protein tyrosine phosphatase, non-receptor type 11) is also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, MGC14433 and belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism(PMID:19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions(PMID:21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1), Noonan syndrome type 1 (NS1), juvenile myelomonocytic leukemia (JMML) and metachondromatosis (MC). It has 3 isoforms produced by alternative splicing.

References

Hagberg N, Theorell J, Schlums H, Eloranta ML, Bryceson YT, Rönnblom L. Systemic lupus erythematosus immune complexes increase the expression of SLAM family members CD319 (CRACC) and CD229 (LY-9) on plasmacytoid dendritic cells and CD319 on CD56(dim) NK cells. Journal of immunology (Baltimore, Md. : 1950). 191(6):2989-98. 2013.
Xiang M, Su H, Hong Z, Yang T, Shu G. Chemical composition of total flavonoids from Polygonum amplexicaule and their pro-apoptotic effect on hepatocellular carcinoma cells: Potential roles of suppressing STAT3 signaling. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 80:62-71. 2015.
Hu ZQ, Ma R, Zhang CM. Expression and clinical significance of tyrosine phosphatase SHP2 in thyroid carcinoma. Oncology letters. 10(3):1507-1512. 2015.

SHP2 antibody

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