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Product Name
SHP1 antibody
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Description
SHP1 Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells, A431 cells. Positive WB detected in HeLa cells, A431 cells, Jurkat cells, Raji cells. Positive IP detected in Jurkat cells. Observed molecular weight by Western-blot: 63 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
HCP antibody; HCPH antibody; HPTP1C antibody; PTP 1C antibody; PTP1C antibody; PTPN6 antibody; SH PTP1 antibody; SHP 1 antibody; SHP 1L antibody; SHP1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SHP1 recombinant protein (Accession Number: NM_080548). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115279(PTPN6 Antibody) at dilution of 1:1000
IP Result of anti-PTPN6 (IP:Catalog No:115279, 4ug; Detection:Catalog No:115279 1:600) with Jurkat cells lysate 3600ug.
Immunofluorescent analysis of MCF-7 cells using Catalog No:115279(PTPN6 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
PTPN6(Tyrosine-protein phosphatase non-receptor type 6) is also named as HCP, PTP1C and belongs to the protein-tyrosine phosphatase family. It regulates muscle insulin action in a cell-autonomous manner, further suggesting that the PTPase negatively modulates insulin action through down-regulation of both insulin signaling to AKT1 and SLC2A4 translocation, as well as SLC2A4 expression(PMID:21952243). It has 4 isoforms produced by alternative splicing.
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References
- Xiang M, Su H, Hong Z, Yang T, Shu G. Chemical composition of total flavonoids from Polygonum amplexicaule and their pro-apoptotic effect on hepatocellular carcinoma cells: Potential roles of suppressing STAT3 signaling. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 80:62-71. 2015.
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