SHMT2 antibody

Cat.#: 115277

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Product Information

  • Product Name
    SHMT2 antibody
  • Documents
  • Description
    SHMT2 Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive IHC detected in human cervical cancer tissue, human heart tissue. Positive WB detected in HeLa cells, HEK-293 cells, MCF7 cells, mouse kidney tissue. Observed molecular weight by Western-blot: 55 kDa
  • Tested applications
    ELISA, WB, IHC, IF
  • Species reactivity
    Human, Mouse, Rat; other species not tested.
  • Alternative names
    GLYA antibody; Serine methylase antibody; SHMT antibody; SHMT2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of SHMT2 recombinant protein (Accession Number: NM_005412). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115277(SHMT2 antibody) at dilution of 1:800

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115277(SHMT2 antibody) at dilution of 1:800

    Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:115277(SHMT2 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:115277(SHMT2 antibody) at dilution of 1:50 (under 10x lens)

    Immunofluorescent analysis of MCF-7 cells using Catalog No:115277(SHMT2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of MCF-7 cells using Catalog No:115277(SHMT2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    SHMT2 represents serine hydroxymethyltransferase 2 (mitochondrial)
  • References
    • Sun L, Song L, Wan Q. cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions. Cell research. 25(4):429-44. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"