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Product Name
SHC antibody
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Description
SHC Rabbit Polyclonal antibody. Positive WB detected in A431 cells, HeLa cells, mouse skin tissue. Positive IHC detected in human prostate cancer tissue. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 66 kDa
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Tested applications
ELISA, IHC, IF, WB
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
FLJ26504 antibody; SH2 domain protein C1 antibody; SHC antibody; SHC transforming protein 1 antibody; SHC transforming protein 3 antibody; SHC transforming protein A antibody; SHC1 antibody; SHCA antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SHC recombinant protein (Accession Number: NM_183001). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:115266(SHC1 Antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:115266(SHC1 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of Hela cells, using SHC1 antibody Catalog No:115266 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
SHC1 represents SHC (Src homology 2 domain containing) transforming protein 1
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