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Product Name
SH3PXD2A antibody
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Description
SH3PXD2A Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive IF detected in HepG2 cells. Positive WB detected in HeLa cells, HepG2 cells. Observed molecular weight by Western-blot: 140-150 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Adapter protein TKS5 antibody; FISH antibody; KIAA0418 antibody; SH3 and PX domains 2A antibody; SH3 multiple domains protein 1 antibody; SH3MD1 antibody; SH3PXD2A antibody; TSK5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_014631). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115262(SH3PXD2A antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:115262(SH3PXD2A antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:115262(SH3PXD2A antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:115262(SH3PXD2A Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
SH3PXD2A is also named as FISH, KIAA0418, SH3MD1, TKS5. SH3PXD2A contains an amino-terminal PX domain followed by five SH3 domains. It is a cytoplasmic protein in normal fibroblasts(PMID:19464300).The p140 and p130 forms of SH3PXD2A may be generated by other splice variations. The complexity of SH3PXD2A isoforms is also evident in different cell types. For example in human platelets a single band of 150kDa was detected, whereas all three forms were found in human fibroblasts and vascular smooth muscle cells(PMID:9687503).This antibody is specific to SH3PXD2A.
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References
- Burger KL, Learman BS, Boucherle AK. Src-dependent Tks5 phosphorylation regulates invadopodia-associated invasion in prostate cancer cells. The Prostate. 74(2):134-48. 2014.
- Blouw B, Patel M, Iizuka S. The invadopodia scaffold protein Tks5 is required for the growth of human breast cancer cells in vitro and in vivo. PloS one. 10(3):e0121003. 2015.
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