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Product Name
SH2D3A antibody
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Description
SH2D3A Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells, HepG2 cells. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 64kd
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Tested applications
ELISA, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
Novel SH2 containing protein 1 antibody; NSP1 antibody; SH2 domain containing 3A antibody; SH2D3A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SH2D3A recombinant protein (Accession Number: NM_005490). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115181(NSP1;SH2D3A antibody) at dilution of 1:1000
Immunofluorescent analysis of HeLa cells using Catalog No:115181(NSP1;SH2D3A Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SH2 domain containing 3A (SH2D3A) Is is a novel SH2-containing protein 1 (NSP1) with proline-rich domains and therefore probably involved in the signal-transduction of some ligand-activated tyrosine kinase receptors. Three phosphorylation sites of NSP1 have been found to date. NSP1 may be located at cell membrane play a role in JNK activation.
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