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SH2D1B antibody

Cat.#: 115179

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Product Information

  • Product Name
    SH2D1B antibody
  • Documents
  • Description
    SH2D1B Rabbit Polyclonal antibody. Positive FC detected in Jurkat cells. Positive IHC detected in human kidney tissue. Positive WB detected in Jurkat cells, HepG2 cells, HL-60 cells, mouse thymus tissue, Raji cells. Observed molecular weight by Western-blot: 15 kDa
  • Tested applications
    ELISA, WB, IHC, FC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    EAT 2 antibody; EAT2 antibody; SH2 domain containing 1B antibody; SH2D1B antibody
  • Immunogen
    Human SH2D1B Recombinant protein (GST tag)
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of SH2D1B recombinant protein (Accession Number: NM_053282). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:115179(SH2D1B antibody) at dilution of 1:800

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:115179(SH2D1B antibody) at dilution of 1:800

    Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:115179(SH2D1B Antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:115179(SH2D1B Antibody) at dilution of 1:50 (under 40x lens)

    1X10^6 Jurkat cells were stained with 0.2ug SH2D1B antibody (Catalog No:115179, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

    1X10^6 Jurkat cells were stained with 0.2ug SH2D1B antibody (Catalog No:115179, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • Background
    SH2D1B, also named as EAT2, an adaptor expressed in innate immune cells, including natural killer (NK) cells. It plays a role in controlling signal transduction through at least four receptors, CD84, SLAMF1, LY9 and CD244, expressed on the surface of professional antigen-presenting cells. EAT-2 overexpression would enhance the innate immune responses and also result in more potent Carcinoembryonic antigen-specific adaptive immune responses.
  • References
    • Hagberg N, Theorell J, Schlums H, Eloranta ML, Bryceson YT, Rönnblom L. Systemic lupus erythematosus immune complexes increase the expression of SLAM family members CD319 (CRACC) and CD229 (LY-9) on plasmacytoid dendritic cells and CD319 on CD56(dim) NK cells. Journal of immunology (Baltimore, Md. : 1950). 191(6):2989-98. 2013.
    • Schlums H, Cichocki F, Tesi B. Cytomegalovirus infection drives adaptive epigenetic diversification of NK cells with altered signaling and effector function. Immunity. 42(3):443-56. 2015.
    • Lee J, Zhang T, Hwang I. Epigenetic modification and antibody-dependent expansion of memory-like NK cells in human cytomegalovirus-infected individuals. Immunity. 42(3):431-42. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"