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SFRP2 antibody

Cat.#: 115145

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Product Information

  • Product Name
    SFRP2 antibody
  • Documents
  • Description
    SFRP2 Rabbit Polyclonal antibody. Positive WB detected in Y79 cells, COLO 320 cells, MCF7 cells. Positive IHC detected in human lung cancer tissue. Observed molecular weight by Western-blot: 33 kDa
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    FRP 2 antibody; FRP2 antibody; FRP-2 antibody; SARP 1 antibody; SARP1 antibody; SDF 5 antibody; sFRP 2 antibody; SFRP2 antibody; UNQ361/PRO697 antibody
  • Immunogen
    Human SFRP2 Recombinant protein (GST tag)
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of SFRP2 recombinant protein (Accession Number: NM_003013). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:115145(SFRP2 antibody) at dilution of 1:400

    Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:115145(SFRP2 antibody) at dilution of 1:400

    Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:115145(SFRP2 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:115145(SFRP2 antibody) at dilution of 1:50 (under 10x lens)

  • Background
    Secreted frizzled-related protein 2 (Sfrp2) is a secreted glycoprotein molecule containing an N terminal cysteine-rich domain, which is typically 30-50% identical to the putative Wnt-binding site of the frizzled receptor, and a C terminal heparin-binding domain with weak homology to netrins. It has been implicated in diverse cellular processes, including embryogenesis, regulation of cell apoptosis, and cell differentiation. Methylation of this gene is a potential marker for the presence of colorectal cancer.
  • References
    • Zhang X, Hao L, Meng L. Digital gene expression tag profiling analysis of the gene expression patterns regulating the early stage of mouse spermatogenesis. PloS one. 8(3):e58680. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"