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Product Name
SFPQ antibody
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Description
SFPQ Rabbit Polyclonal antibody. Positive IHC detected in human brain tissue. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells, BxPC-3 cells, human brain tissue, Jurkat cells, mouse brain tissue, PC-3 cells, Y79 cells. Observed molecular weight by Western-blot: 100 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
100 kDa DNA pairing protein antibody; hPOMp100 antibody; POMP100 antibody; PSF antibody; PTB assOCiated splicing factor antibody; SFPQ antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SFPQ recombinant protein (Accession Number: NM_005066). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115144(SFPQ antibody) at dilution of 1:700
Immunohistochemical of paraffin-embedded human brain using Catalog No:115144(SFPQ antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:115144(SFPQ antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:115144(SFPQ Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SFPQ, also named PSF, encodes a nuclear factor implicated in the splicing and regulation of gene expression. SFPQ probably forms a heteromer with NONO and participates in DNA pairing and DNA break repair program. Very recently SFPQ was identified as a downstream target of tau, complete nuclear depletion and cytoplasmic accumulation of SFPQ were shown in the neurons and astrocytes of brains with Alzheimer's disease (AD), more strikingly, reduced SFPQ levels may progress together with tau pathology, these observation strongly suggests the important role of SFPQ pathology in neurodegenerative diseases including AD. SFPQ protein may appears as 47 kDa, 52 kDa or 100 kDa molecules in various cell types.This a rabbit polyclonal antibody raised against the N-terminal of human SFPQ.
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References
- Huang R, Yu M, Li CY. New insights into the functions and localization of nuclear CCT protein complex in K562 leukemia cells. Proteomics. Clinical applications. 6(9-10):467-75. 2012.
- Grebeňová D, Röselová P, Pluskalová M. Proteins implicated in the increase of adhesivity induced by suberoylanilide hydroxamic acid in leukemic cells. Journal of proteomics. 77:406-22. 2012.
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