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Product Name
SF3B3 antibody
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Description
SF3B3 Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive IHC detected in human gliomas tissue. Positive IP detected in rat brain tissue. Positive WB detected in HeLa cells, human brain tissue, mouse heart tissue, rat brain tissue, rat heart tissue. Observed molecular weight by Western-blot: 130kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
KIAA0017 antibody; RSE1 antibody; SAP 130 antibody; SAP130 antibody; SF3b130 antibody; SF3B3 antibody; Splicing factor 3B subunit 3 antibody; STAF130 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SF3B3 recombinant protein (Accession Number: BC003146). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:2000-1:20000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115138(SF3B3 antibody) at dilution of 1:10000
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:115138(SF3B3 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using SF3B3 antibody Catalog No:115138 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-SF3B3 (IP:Catalog No:115138, 4ug; Detection:Catalog No:115138 1:5000) with rat brain tissue lysate 4800ug.
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Background
Introns are removed from nuclear pre-mRNA in 2-step transesterification reactions. Splicing occured in a large ribonucleoprotein particle, called the spliceosome. Spliceosomal intermediate complexes form on pre-mRNA in the order E, A, B, and C, with the catalytic reactions occurring in complex C. U2 small nuclear ribonucleoproteins are one of the proteins essential for spliceosome assembly and mRNA splicing. Functional U2 snRNP is composed of a 12S unit and 2 splicing factors, SF3A, which is composed of 3 proteins, and SF3B, which conposed of 4 proteins. SF3B3 is one of SF3B, and it's required for 'A' complex assembly formed by the stable binding of U2 snRNP to the brachpoint sequence(BPS) in pre-mRNA.
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References
- Coltri P, Effenberger K, Chalkley RJ, Burlingame AL, Jurica MS. Breaking up the C complex spliceosome shows stable association of proteins with the lariat intron intermediate. PloS one. 6(4):e19061. 2011.
- Ilagan JO, Chalkley RJ, Burlingame AL, Jurica MS. Rearrangements within human spliceosomes captured after exon ligation. RNA (New York, N.Y.). 19(3):400-12. 2013.
- Kfir N, Lev-Maor G, Glaich O. SF3B1 association with chromatin determines splicing outcomes. Cell reports. 11(4):618-29. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"