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Product Name
SF3A1 antibody
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Description
SF3A1 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive IF detected in HepG2 cells. Positive WB detected in K-562 cells, HEK-293 cells, HeLa cells, HL-60 cells, mouse brain tissue, mouse lung tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 120-130 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
PRP21 antibody; PRPF21 antibody; SAP 114 antibody; SAP114 antibody; SF3A1 antibody; SF3A120 antibody; Splicing factor 3A subunit 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SF3A1 recombinant protein (Accession Number: NM_005877). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:115133(SF3A1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:115133(SF3A1 antibody) at dilution of 1:400 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:115133(SF3A1 antibody) at dilution of 1:400 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using SF3A1 antibody Catalog No:115133 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-SF3A1 (IP:Catalog No:115133, 4ug; Detection:Catalog No:115133 1:500) with mouse brain tissue lysate 4000ug.
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Background
SF3A1 represents splicing factor 3a, subunit 1, 120kDa
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References
- Kfir N, Lev-Maor G, Glaich O. SF3B1 association with chromatin determines splicing outcomes. Cell reports. 11(4):618-29. 2015.
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