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Product Name
SETDB1 antibody
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Description
SETDB1 Rabbit Polyclonal antibody. Positive WB detected in MCF7 cells, HEK-293 cells, human testis tissue, Jurkat cells, mouse brain tissue, PC-3 cells. Positive IP detected in MCF-7 cells. Positive IHC detected in human testis tissue. Observed molecular weight by Western-blot: 170 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
ESET antibody; H3 K9 HMTase 4 antibody; H3 K9 HMTase4 antibody; KG1T antibody; KIAA0067 antibody; KMT1E antibody; Lysine N methyltransferase 1E antibody; SET domain bifurcated 1 antibody; SET domain antibody; bifurcated 1 antibody; SETDB1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SETDB1 recombinant protein (Accession Number: NM_001393964). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:115130(SETDB1 antibody) at dilution of 1:1000
IP Result of anti-SETDB1 (IP:Catalog No:115130, 3ug; Detection:Catalog No:115130 1:1000) with MCF-7 cells lysate 2500ug.
Immunohistochemical of paraffin-embedded human testis using Catalog No:115130(SETDB1 antibody) at dilution of 1:50 (under 40x lens)
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Background
SETDB1, also named as ESET, KIAA0067 and KMT1E, belongs to the histone-lysine methyltransferase family. It is a SET domain protein with histone H3-K9-specific methyltransferase activity. H3 'Lys-9' trimethylation is coordinated with DNA methylation and represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. SETDB1 mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 regulates histone methylation, gene silencing, and transcriptional repression. It has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SETDB1.
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References
- Kera Y, Katoh Y, Ohta M, Matsumoto M, Takano-Yamamoto T, Igarashi K. Methionine adenosyltransferase II-dependent histone H3K9 methylation at the COX-2 gene locus. The Journal of biological chemistry. 288(19):13592-601. 2013.
- Sun QY, Ding LW, Xiao JF. SETDB1 accelerates tumourigenesis by regulating the WNT signalling pathway. The Journal of pathology. 235(4):559-70. 2015.
- Martin FJ, Xu Y, Lohmann F. KMT1E-mediated chromatin modifications at the FcγRIIb promoter regulate thymocyte development. Genes and immunity. 16(2):162-9. 2015.
- Collins PL, Kyle KE, Egawa T, Shinkai Y, Oltz EM. The histone methyltransferase SETDB1 represses endogenous and exogenous retroviruses in B lymphocytes. Proceedings of the National Academy of Sciences of the United States of America. 112(27):8367-72. 2015.
- Bilodeau S, Kagey MH, Frampton GM, Rahl PB, Young RA. SetDB1 contributes to repression of genes encoding developmental regulators and maintenance of ES cell state. Genes & development. 23(21):2484-9. 2009.
- Cruvinel E, Budinetz T, Germain N, Chamberlain S, Lalande M, Martins-Taylor K. Reactivation of maternal SNORD116 cluster via SETDB1 knockdown in Prader-Willi syndrome iPSCs. Human molecular genetics. 23(17):4674-85. 2014.
- An J, Zhang X, Qin J. The histone methyltransferase ESET is required for the survival of spermatogonial stem/progenitor cells in mice. Cell death & disease. 5:e1196. 2014.
- Frietze S, O'Geen H, Blahnik KR, Jin VX, Farnham PJ. ZNF274 recruits the histone methyltransferase SETDB1 to the 3' ends of ZNF genes. PloS one. 5(12):e15082. 2010.
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