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Sestrin 2 antibody

Cat.#: 115217

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Product Information

  • Product Name
    Sestrin 2 antibody
  • Documents
  • Description
    Sestrin 2 Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive WB detected in mouse thymus tissue, HeLa cells, K-562 cells, mouse lung tissue, NIH/3T3 cells. Observed molecular weight by Western-blot: 60-66 kDa
  • Tested applications
    ELISA, WB, IF
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    DKFZp761M0212 antibody; DKFZp761M02121 antibody; HI95 antibody; SES2 antibody; SESN2 antibody; SEST2 antibody; Sestrin 2 antibody; Sestrin2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Sestrin 2 recombinant protein (Accession Number: NM_031459). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:1000-1:10000

    IF: 1:10-1:100

  • Validations

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with Catalog No:115217(SESN2 antibody) at dilution of 1:500

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with Catalog No:115217(SESN2 antibody) at dilution of 1:500

    Immunofluorescent analysis of Hela cells, using SESN2 antibody Catalog No:115217 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of Hela cells, using SESN2 antibody Catalog No:115217 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Background
    Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 2 is implicated in the regulation of cell growth and survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy. Sestrin 2 is approximately a 57.6kDa protein (480 amino acids).
  • References
    • Hou YS, Guan JJ, Xu HD, Wu F, Sheng R, Qin ZH. Sestrin2 Protects Dopaminergic Cells against Rotenone Toxicity through AMPK-Dependent Autophagy Activation. Molecular and cellular biology. 35(16):2740-51. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"