Human STK4/MST1 (His Tag) recombinant protein

Human STK4/MST1 (His Tag) recombinant protein

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Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation (By similarity). Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation. Phosphorylates MOBKL1A, MOBKL1B and RASSF2. Phosphorylates TNNI3 (cardiac Tn-I) and alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). Phosphorylates FOXO1 on 'Ser-212' and regulates its activation and stimulates transcription of PMAIP1 in a FOXO1-dependent manner. Phosphorylates SIRT1 and inhibits SIRT1-mediated p53/TP53 deacetylation, thereby promoting p53/TP53 dependent transcription and apoptosis upon DNA damage. Acts as an inhibitor of PKB/AKT1. Phosphorylates AR on 'Ser-650' and suppresses its activity by intersecting with PKB/AKT1 signaling and antagonizing formation of AR-chromatin complexes.

Serine/threonine-protein kinase 4

MST1; KRS2; MST-1; KAS-2; AU020804; AI447339; STK4; YSK3; RP23-346D16.3; DKFZP686A2068; TIIAC

Q13043

STK4; KRS2; MST1

Baculovirus-Insect Cells

A DNA sequence encoding the human STK4 isoform 1 (Q13043-1) (Glu 2-Phe 487) was expressed, with a polyhistidine tag at the N-terminus.

Human

The recombinant human STK4 consists of 505 amino acids and migrates as an approximately 58 kDa band in SDS-PAGE under reducing conditions as predicted.

> 92 % as determined by SDS-PAGE

The specific activity was determined to be 253 nmol/min/mg using MBP as substrate.