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Product Name
SEC61G antibody
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Description
SEC61G Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 7.7 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Sec61 gamma subunit antibody; SEC61G antibody; SSS1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SEC61G recombinant protein (Accession Number: NM_014302). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115089(SEC61G antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:115089(SEC61G antibody) at dilution of 1:50 (under 10x lens)
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Background
SEC61G is a subunit of the SEC61 complex, which also contains alpha (SEC61A) and beta (SEC61B) subunits. The SEC61 complex is the central component of the protein translocation apparatus on the endoplasmic reticulum membrane. The gene of SEC61G maps to chromosome 7p11.2, and encodes a 68-amino acid single-pass membrane protein. This antibody can recognize endogenous SEC61G that migrates at a molecular mass of ~8 kDa.
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References
- Lu Z, Zhou L, Killela P. Glioblastoma proto-oncogene SEC61gamma is required for tumor cell survival and response to endoplasmic reticulum stress. Cancer research. 69(23):9105-11. 2009.
- Zehner M, Marschall AL, Bos E. The translocon protein Sec61 mediates antigen transport from endosomes in the cytosol for cross-presentation to CD8(+) T cells. Immunity. 42(5):850-63. 2015.
- Tsukamoto Y, Uchida T, Karnan S. Genome-wide analysis of DNA copy number alterations and gene expression in gastric cancer. The Journal of pathology. 216(4):471-82. 2008.
- Saito K, Dubreuil V, Arai Y. Ablation of cholesterol biosynthesis in neural stem cells increases their VEGF expression and angiogenesis but causes neuron apoptosis. Proceedings of the National Academy of Sciences of the United States of America. 106(20):8350-5. 2009.
- Mackinnon AL, Paavilainen VO, Sharma A, Hegde RS, Taunton J. An allosteric Sec61 inhibitor traps nascent transmembrane helices at the lateral gate. eLife. 3:e01483. 2014.
- Johnson GG, White MC, Wu JH, Vallejo M, Grimaldi M. The deadly connection between endoplasmic reticulum, Ca2+, protein synthesis, and the endoplasmic reticulum stress response in malignant glioma cells. Neuro-oncology. 16(8):1086-99. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"