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Product Name
SEC61B antibody
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Description
SEC61B Rabbit Polyclonal antibody. Positive IF detected in HeLa cells, HepG2 cells. Positive IHC detected in human cervical cancer tissue. Positive WB detected in mouse liver tissue, HeLa cells, HepG2 cells, L02 cells. Positive IP detected in HepG2 cells. Observed molecular weight by Western-blot: 12-15 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
Sec61 beta subunit antibody; SEC61B antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SEC61B recombinant protein (Accession Number: NM_006808). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:115086(SEC61B antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:115086(SEC61B antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:115086(SEC61B antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-SEC61B (IP:Catalog No:115086, 4ug; Detection:Catalog No:115086 1:500) with HepG2 cells lysate 1800ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:115086(SEC61B Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
The heteromeric SEC61 complex is composed of alpha, beta and gamma subunits. Oligomers of the SEC61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane (PMID: 10212142). SEC61B is the beta subunit of the SEC61 complex. It has been reported that SEC61B facilitates cotranslational protein transport and interacts with the signal peptidase during translocation (PMID: 9585408).
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References
- Norlin S, Parekh VS, Naredi P, Edlund H. Asna1/TRC40 Controls β-Cell Function and Endoplasmic Reticulum Homeostasis by Ensuring Retrograde Transport. Diabetes. 65(1):110-9. 2016.
- Ninagawa S, Okada T, Sumitomo Y. Forcible destruction of severely misfolded mammalian glycoproteins by the non-glycoprotein ERAD pathway. The Journal of cell biology. 211(4):775-84. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"