Product Name

SEC61alpha antibody

Documents

Description

SEC61alpha Rabbit Polyclonal antibody. Positive WB detected in mouse thymus tissue, mouse liver tissue. Positive IHC detected in human liver tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 70 kDa

Tested applications

ELISA, WB, IHC, IF

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

HSEC61 antibody; SEC61 antibody; Sec61 alpha 1 antibody; SEC61A antibody; SEC61A1 antibody; SEC61alpha antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of SEC61alpha recombinant protein (Accession Number: BC002951). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:200-1:2000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

Immunofluorescent analysis of HepG2 cells, using SEC61A1 antibody Catalog No:115085 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

mouse thymus tissue were subjected to SDS PAGE followed by western blot with Catalog No:115085(SEC61A1 antibody) at dilution of 1:200

Immunohistochemical of paraffin-embedded human liver using Catalog No:115085(SEC61A1 antibody) at dilution of 1:100 (under 40x lens)

Immunohistochemical of paraffin-embedded human liver using Catalog No:115085(SEC61A1 antibody) at dilution of 1:100 (under 10x lens)

Background

The SEC61 complex is a major component of the endoplasmic reticulum (ER) translocation site (PMID: 7720858). This complex is composed of three subunits: SEC61alpha, SEC61beta and SEC61gamma. SEC61alpha is a polytopic integral membrane protein. It is a core component of the ER translocation complex, playing a crucial role in the insertion of secretory and membrane polypeptides into the ER (PMID: 9512475).

SEC61alpha antibody

Product Information

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