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SEC5/EXOC2 antibody

Cat.#: 107522

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Product Information

  • Product Name
    SEC5/EXOC2 antibody
  • Documents
  • Description
    SEC5/EXOC2 Mouse Monoclonal antibody. Positive IF detected in Hela cells. Positive WB detected in Human brain. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 100 kDa
  • Tested applications
    ELISA, WB, IF, IP
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    EXOC2 antibody; exOCyst complex component 2 antibody; ExOCyst complex component Sec5 antibody; FLJ11026 antibody; SEC5 antibody; SEC5L1 antibody; Sec5p antibody
  • Isotype
    Mouse IgG2b
  • Preparation
    This antibody was obtained by immunization of SEC5/EX℃2 recombinant protein (Accession Number: BC016918). Purification method: Protein A purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IF: 1:10-1:100

  • Validations

    human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:107522(SEC5 antibody) at dilution of 1:1000

    human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:107522(SEC5 antibody) at dilution of 1:1000

    Immunofluorescent analysis of Hela cells, using EXoc2 antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    Immunofluorescent analysis of Hela cells, using EXoc2 antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    IP Result of anti-SEC5 (IP:Catalog No:107522, 5ug; Detection:Catalog No:107522 1:500) with mouse brain tissue lysate 3440ug.

    IP Result of anti-SEC5 (IP:Catalog No:107522, 5ug; Detection:Catalog No:107522 1:500) with mouse brain tissue lysate 3440ug.

  • Background
    EXoc2 (exocyst complex component 2), also known as SEC5 and SEC5L1, is a component of the exocyst complex, and is required to mediate RalB-dependent survival signals in transformed cells. The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is involved in tethering post-Golgi secretory vesicles to specific plasma membrane domains. The gene of EXoc2 maps to chromosome 6p25.3, and encodes a 924-amino acid protein with an experimentally determined molecular mass of 95-100 kDa. EXoc2 mRNA is widely expressed with highest levels in brain and placenta.
  • References
    • Gonzalez IM, Ackerman WE, Vandre DD, Robinson JM. Exocyst complex protein expression in the human placenta. Placenta. 35(7):442-9. 2014.
    • Fahmy K, Gonzalez A, Arafa M. Myoferlin plays a key role in VEGFA secretion and impacts tumor-associated angiogenesis in human pancreas cancer. International journal of cancer. 138(3):652-63. 2016.
    • Wang HL, Yang CH, Lee HH. Dexamethasone-induced cellular tension requires a SGK1-stimulated Sec5-GEF-H1 interaction. Journal of cell science. 128(20):3757-68. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"