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Product Name
SEC23IP antibody
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Description
SEC23IP Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells. Positive IF detected in HeLa cells. Positive IHC detected in human heart tissue, human liver tissue. Observed molecular weight by Western-blot: 125 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
MSTP053 antibody; P125 antibody; SEC23 interacting protein antibody; SEC23IP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SEC23IP recombinant protein (Accession Number: XM_047424537). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115078(SEC23IP antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human heart using Catalog No:115078(SEC23IP antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:115078(SEC23IP antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-SEC23IP (IP:Catalog No:115078, 4ug; Detection:Catalog No:115078 1:1000) with HeLa cells lysate 3600ug.
Immunofluorescent analysis of HeLa cells using Catalog No:115078(SEC23IP Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SEC23-interacting protein (SEC23IP), also known as p125, is a member of the phosphatidic acid preferring-phospholipase A1 family. SEC23IP interacts with Sec23, a coat component of COPII vesicles which are involved in protein export from the endoplasmic reticulum (ER). SEC23IP localizes to ER exit sites and participates in the organization of this compartment (PMID: 15623529). It may have a role in the early stage of the secretory pathway (PMID: 10400679).
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