-
Product Name
SEC13 antibody
- Documents
-
Description
SEC13 Rabbit Polyclonal antibody. Positive IHC detected in mouse brain tissue, human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue. Positive IF detected in HepG2 cells. Positive WB detected in HepG2 cells, RAW 264.7 cells. Positive IP detected in HepG2 cells. Observed molecular weight by Western-blot: 36 kDa
-
Tested applications
ELISA, WB, IHC, IF, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
D3S1231E antibody; Protein SEC13 homolog antibody; SEC13 antibody; SEC13 homolog (S. cerevisiae) antibody; SEC13 like protein 1 antibody; SEC13 related protein antibody; SEC13L1 antibody; SEC13R antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of SEC13 recombinant protein (Accession Number: XM_047448692). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:115075(SEC13 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:115075(SEC13 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:115075(SEC13 Antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-SEC13 (IP:Catalog No:115075, 3ug; Detection:Catalog No:115075 1:1000) with HepG2 cells lysate 3200ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:115075(SEC13 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
SEC13 is a component of the nuclear pore complex (NPC) and the COPII coat. It is required for esicle biogenesis from endoplasmic reticulum during the transport of proteins. Shuttling of SEC13 between the nucleus and the cytoplasm may couple and regulate functions between these two compartments. (PMID: 14517296; 16407955)
-
References
- Teodorof C, Divakar S, Soontornniyomkij B, Achim CL, Kaul M, Singh KK. Intracellular mannose binding lectin mediates subcellular trafficking of HIV-1 gp120 in neurons. Neurobiology of disease. 69:54-64. 2014.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"