-
Product Name
SCRN3 antibody
- Documents
-
Description
SCRN3 Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue, human pancreas tissue. Positive IF detected in HepG2 cells. Positive WB detected in HepG2 cells, A549 cells, HEK-293 cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 49kd
-
Tested applications
ELISA, WB, IP, IHC, IF
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
FLJ23142 antibody; SCRN3 antibody; secernin 3 antibody; SES3 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of SCRN3 recombinant protein (Accession Number: BC031821). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:115021(SCRN3 antibody) at dilution of 1:500
IP Result of anti-SCRN3 (IP:Catalog No:115021, 4ug; Detection:Catalog No:115021 1:800) with HEK-293 cells lysate 1760ug.
Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:115021(SCRN3 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:115021(SCRN3 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:115021(SCRN3 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
SCRN genes encode protein members of the secernin family including Secernin 1, Secernin 2 and Secernin 3. Function of Secernin 2 and Secernin 3 are so far not well understood. Secernin 1 is a novel 50-kDa cytosolic protein that appears to be involved in the regulation of exocytosis from peritoneal mast cells. Secernin-1 a novel tumor-associated antigen (TAA) and may be a universal marker of different cancer types including gastric cancer, which has been validated by several researches. Measurement of SCRN1 can be used in the early detection of cancer or in the surveillance of patients who undergo surgery.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"