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Product Name
SCML2 antibody
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Description
SCML2 Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue, human placenta tissue. Positive IF detected in HEK-293 cells. Positive WB detected in K-562 cells, HEK-293 cells, mouse testis tissue. Observed molecular weight by Western-blot: 70 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
SCML2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SCML2 recombinant protein (Accession Number: NM_006089). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:115003(SCML2 Antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:115003(SCML2 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:115003(SCML2 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HEK-293 cells using Catalog No:115003(SCML2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
The human SCML2 gene, a mammalian homologue of the Drosophila PcG protein SCM, encodes two protein isoforms: SCML2A that is bound to chromatin and SCML2B that is predominantly nucleoplasmic (PMID: 24358021). Polycomb repressive complex-1 (PRC1) is essential for the epigenetic regulation of gene expression. SCML2 is a Polycomb-group protein that associates with PRC1. SCML2 participates in the epigenetic control of transcription directly and in cooperation with PRC1 (PMID: 24986859).
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