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Product Name
SCAP antibody
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Description
SCAP Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, K-562 cells. Positive IHC detected in human lung cancer tissue, human kidney tissue. Positive IF detected in HEK-293 cells. Observed molecular weight by Western-blot: 140kd,96kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
KIAA0199 antibody; SCAP antibody; SREBF chaperone antibody; SREBP cleavage-activating protein antibody; Sterol regulatory element-binding protein cleavage-activating protein antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SCAP recombinant protein (Accession Number: BC020987). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:114984(SCAP antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:114984(SCAP Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:114984(SCAP Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HEK-293 cells using Catalog No:114984(SCAP Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SCAP is a regulatory protein that is required for the proteolytic cleavage of the sterol regulatory element-binding protein (SREBP). SCAP is an integral membrane protein located in the endoplasmic reticulum (ER). One of the cytosolic regions of SCAP contains a hexapeptide amino acid sequence, MELADL, that functions to detect cellular cholesterol. When cholesterol is present, SCAP undergoes a conformational change that prevents it from activating SREBP and cholesterol synthesis does not occur.
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References
- Liew CW, Assmann A, Templin AT. Insulin regulates carboxypeptidase E by modulating translation initiation scaffolding protein eIF4G1 in pancreatic β cells. Proceedings of the National Academy of Sciences of the United States of America. 111(22):E2319-28. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"