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Product Name
SACM1L antibody
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Description
SACM1L Rabbit Polyclonal antibody. Positive WB detected in human kidney tissue, 3T3 cells (RNAi), human brain tissue, mouse kidney tissue, mouse lung tissue. Positive IP detected in mouse kidney tissue. Positive IHC detected in human brain tissue, human heart tissue. Observed molecular weight by Western-blot: 60-67 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp686A0231 antibody; KIAA0851 antibody; SAC1 antibody; SACM1L antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SACM1L recombinant protein (Accession Number: NM_014016). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
WB result of anti- SAC1 (Catalog No:115041) with NIH-3T3 cells (RNAi).
human kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:115041(SACM1L antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human brain using Catalog No:115041(SACM1L antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:115041(SACM1L antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-SACM1L (IP:Catalog No:115041, 4ug; Detection:Catalog No:115041 1:1000) with mouse kidney tissue lysate 4000ug.
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Background
SACM1L(Suppressor of actin mutations 1-like protein) is also named as KIAA0851.It is a phosphoinositide phosphatase that hydrolyzes PtdIns3P and PtdIns4P and has low activity towards PtdIns(3,5)P2.
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References
- Tokuda E, Itoh T, Hasegawa J. Phosphatidylinositol 4-phosphate in the Golgi apparatus regulates cell-cell adhesion and invasive cell migration in human breast cancer. Cancer research. 74(11):3054-66. 2014.
- Yang G, Zhou X, Zhu J. JNK3 couples the neuronal stress response to inhibition of secretory trafficking. Science signaling. 6(283):ra57. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"