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Product Name
S6K2 antibody
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Description
S6K2 Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive IP detected in MCF-7 cells. Positive WB detected in MCF7 cells. Observed molecular weight by Western-blot: 54 kDa
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
KLS antibody; P70 beta antibody; P70 beta 1 antibody; P70 beta 2 antibody; p70 ribosomal S6 kinase beta antibody; P70 S6 kinase beta antibody; p70 S6K 2 antibody; p70 S6K beta antibody; p70 S6KB antibody; p70(S6K) beta antibody; P70S6K2 antibody; p70S6Kb antibody; RPS6KB2 antibody; S6 kinase related kinase antibody; S6K beta antibody; S6K beta 2 antibody; S6K beta2 antibody; S6K2 antibody; SRK antibody; STK14B antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of S6K2 recombinant protein (Accession Number: NM_003952). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IF: 1:50-1:500
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:115036(RPS6KB2 antibody) at dilution of 1:1500
IP Result of anti-RPS6KB2 (IP:Catalog No:115036, 4ug; Detection:Catalog No:115036 1:1000) with MCF-7 cells lysate 2800ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using Catalog No:115036(RPS6KB2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
RPS6KB2(ribosomal protein S6 kinase beta-2) is also named as 70 kDa ribosomal protein S6 kinase 2(P70S6K2),Serine/threonine-protein kinase 14B(STK14B) and belongs to the protein kinase superfamily. It is activated by mitogenic stimuli, including growth factors, cytokines, and phorbol esters. The activated enzyme phosphorylates several serine residues in the C terminus of ribosomal protein S6(PMID:9878560).
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