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Product Name
S100A5 antibody
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Description
S100A5 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, human kidney tissue, human liver tissue, SH-SY5Y cells. Positive IF detected in HepG2 cells. Positive IHC detected in human brain tissue. Observed molecular weight by Western-blot: 11 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Protein S 100D antibody; Protein S100 A5 antibody; S100A5 antibody; S100D antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of S100A5 recombinant protein (Accession Number: XM_017002030). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:114962(S100A5 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human brain using Catalog No:114962(S100A5 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:114962(S100A5 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:114962(S100A5 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
S100A5 represents S100 calcium binding protein A5
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References
- Kuhlmann K, Tschapek A, Wiese H. The membrane proteome of sensory cilia to the depth of olfactory receptors. Molecular & cellular proteomics : MCP. 13(7):1828-43. 2014.
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