-
Product Name
S100A10 antibody
- Documents
-
Description
S100A10 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human lung cancer tissue, human gliomas tissue, human pancreas cancer tissue, human skin tissue. Positive FC detected in HeLa cells. Positive WB detected in mouse lung tissue, human lung tissue, mouse small intestine tissue, rat skin tissue. Observed molecular weight by Western-blot: 11 kDa
-
Tested applications
ELISA, WB, FC, IF, IHC
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
42C antibody; ANX2L antibody; ANX2LG antibody; Ca[1] antibody; CAL1L antibody; Calpactin 1 light chain antibody; Calpactin I light chain antibody; Cellular ligand of annexin II antibody; CLP11 antibody; GP11 antibody; p10 antibody; p10 protein antibody; P11 antibody; Protein S100 A10 antibody; S100A10 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of S100A10 recombinant protein (Accession Number: NM_002966). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:100-1:1000
IF: 1:20-1:200
-
Validations
mouse lung tissue were subjected to SDS PAGE followed by western blot with Catalog No:114954(S100A10 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:114954(S100A10 Antibody) at dilution of 1:400 (under 10x lens).
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:114954(S100A10 Antibody) at dilution of 1:400 (under 40x lens).
Immunofluorescent analysis of HeLa cells using Catalog No:114954(S100A10 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HeLa cells were stained with 0.2ug S100A10 antibody (Catalog No:114954, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
-
Background
S100A10, also known as p11, is a member of the S100 family of small, EF hand containing dimeric proteins. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A10 is present on the surface of endothelial and other cells in a heterotetrameric complex with another Ca(2+)-binding protein, annexin II. S100A10 may function in exocytosis and endocytosis.
-
References
- He KL, Sui G, Xiong H. Feedback regulation of endothelial cell surface plasmin generation by PKC-dependent phosphorylation of annexin A2. The Journal of biological chemistry. 286(17):15428-39. 2011.
- Zhou X, Cui Y, Luan J. Label-free quantification proteomics reveals novel calcium binding proteins in matrix vesicles isolated from mineralizing Saos-2 cells. Bioscience trends. 7(3):144-51. 2013.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"