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Product Name
RYBP antibody
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Description
RYBP Rabbit Polyclonal antibody. Positive IP detected in mouse spleen tissue. Positive WB detected in K-562 cells. Observed molecular weight by Western-blot: 32 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AAP1 antibody; APAP 1 antibody; Apoptin assOCiating protein 1 antibody; DED assOCiated factor antibody; DEDAF antibody; RING1 and YY1 binding protein antibody; RYBP antibody; YEAF1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RYBP recombinant protein (Accession Number: NM_012234). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:114950(RYBP antibody) at dilution of 1:500
IP Result of anti-RYBP (IP:Catalog No:114950, 4ug; Detection:Catalog No:114950 1:300) with mouse spleen tissue lysate 4000ug.
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Background
Polycomb repressive complexes (PRCs) are important chromatin regulators of embryonic stem (ES) cell function [PMID:22269950]. RYBP binds Polycomb H2A monoubiquitin ligases Ring1A and Ring1B and has been suggested to assist PRC localization to their targets. RYBP, firstly identified as a direct interactor with Ring1A, acts as transcriptional repressor in reporter assays, both in tissue culture cells and in the fly [PMID:16125914]
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