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Product Name
RWDD1 antibody
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Description
RWDD1 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, HeLa cells, human skeletal muscle tissue, mouse thymus tissue. Positive IP detected in mouse thymus tissue. Observed molecular weight by Western-blot: 28 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CGI 24 antibody; DFRP2 antibody; PTD013 antibody; RWD domain containing 1 antibody; RWDD1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RWDD1 recombinant protein (Accession Number: NM_015952). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:114941(RWDD1 antibody) at dilution of 1:400
IP Result of anti-RWDD1 (IP:Catalog No:114941, 4ug; Detection:Catalog No:114941 1:300) with mouse thymus tissue lysate 2800ug.
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Background
RWDD1(RWD domain-containing protein 1) is also named as DFRP2(DRG family-regulatory protein 2) and belongs to the RWDD1/GIR2 family. RWDD1 is a thymus aging-related protein which contains an RWD domain at its N-terminus and it is expressed in both thymocytes and thymic epithelial cells and located primarily in the cytoplasm. This protein expression in thymus is decreased in aged and oxidatively stressed mice(PMID:18954556).
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References
- Grötsch H, Kunert M, Mooslehner KA. RWDD1 interacts with the ligand binding domain of the androgen receptor and acts as a coactivator of androgen-dependent transactivation. Molecular and cellular endocrinology. 358(1):53-62. 2012.
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