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Product Name
RUVBL2 antibody
- Documents
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Description
RUVBL2 Rabbit Polyclonal antibody. Positive WB detected in K-562 cells, mouse testis tissue, rat testis tissue. Positive IP detected in HeLa cells, mouse brain tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human testis tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue. Observed molecular weight by Western-blot: 51kd
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
48 kDa TBP interacting protein antibody; CGI 46 antibody; ECP 51 antibody; ECP51 antibody; INO80 complex subunit J antibody; INO80J antibody; Repressing pontin 52 antibody; REPTIN antibody; Reptin 52 antibody; RuvB like 2 antibody; RuvB like 2 (E. coli) antibody; RUVBL2 antibody; RVB2 antibody; TAP54 beta antibody; TIH2 antibody; TIP48 antibody; TIP49B antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RUVBL2 recombinant protein (Accession Number: NM_006666). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:114940(RUVBL2 antibody) at dilution of 1:800
IP result of anti-RUVBL2(Catalog No:114940 for IP and Detection).
Immunohistochemical of paraffin-embedded human testis using Catalog No:114940(RUVBL2 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:114940(RUVBL2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
RuvB-like 2 (E. coli) (RUVBL2, synonyms: ECP51, TIP48, CGI-46, TIP49B) is the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that RUVBL2 has both ATPase and DNA helicase activities.
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References
- Boulon S, Marmier-Gourrier N, Pradet-Balade B. The Hsp90 chaperone controls the biogenesis of L7Ae RNPs through conserved machinery. The Journal of cell biology. 180(3):579-95. 2008.
- Machado-Pinilla R, Liger D, Leulliot N, Meier UT. Mechanism of the AAA+ ATPases pontin and reptin in the biogenesis of H/ACA RNPs. RNA (New York, N.Y.). 18(10):1833-45. 2012.
- Bizarro J, Charron C, Boulon S. Proteomic and 3D structure analyses highlight the C/D box snoRNP assembly mechanism and its control. The Journal of cell biology. 207(4):463-80. 2014.
- Kato M, Chou TF, Yu CZ, DeModena J, Sternberg PW. LINKIN, a new transmembrane protein necessary for cell adhesion. eLife. 3:e04449. 2014.
- Kim SG, Hoffman GR, Poulogiannis G. Metabolic stress controls mTORC1 lysosomal localization and dimerization by regulating the TTT-RUVBL1/2 complex. Molecular cell. 49(1):172-85. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"