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Product Name
RUNX1 antibody
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Description
RUNX1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in mouse thymus tissue, Jurkat cells. Positive IP detected in Jurkat cells. Observed molecular weight by Western-blot: 49 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
AML1 antibody; AML1 EVI 1 antibody; AMLCR1 antibody; CBF alpha 2 antibody; CBFA2 antibody; EVI 1 antibody; Oncogene AML 1 antibody; PEA2 alpha B antibody; PEBP2 alpha B antibody; PEBP2aB antibody; RUNX1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RUNX1 recombinant protein (Accession Number: XM_047441007). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IF: 1:10-1:100
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Validations
mouse thymus tissue were subjected to SDS PAGE followed by western blot with Catalog No:114936(RUNX1 Antibody) at dilution of 1:1000
IP Result of anti-RUNX1 (IP:Catalog No:114936, 4ug; Detection:Catalog No:114936 1:300) with Jurkat cells lysate 3440ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:114936(RUNX1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Runt-related transcription factor 1 (RUNX1), also named as AML1 or CBF alpha 2, is 453 amino acid protein, which contains one Runt domain. RUNX1 localizes in the nucleus and is expressed in all tissues except brain and heart. RUNX1 is involved in hematopoiesis and is frequently targeted in human leukemia by chromosomal translocations that fuse the DNA-binding domain of RUNX1 to other transcription factors and corepressor molecules. In addition to its role in leukemogenesis, RUNX1 is also involved in sensory neuron diversification.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"