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Product Name
RTN4/NOGO antibody
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Description
RTN4/NOGO Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue. Positive IHC detected in human prostate cancer tissue. Positive FC detected in SH-SY5Y cells. Observed molecular weight by Western-blot: 45 kDa, 190-210 kDa
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Tested applications
ELISA, WB, IHC, FC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
ASY antibody; FoOCen antibody; KIAA0886 antibody; Nbla00271 antibody; Nbla10545 antibody; Neurite outgrowth inhibitor antibody; NI220/250 antibody; NOGO antibody; NOGO A antibody; Nogo B antibody; Nogo C antibody; Nogo protein antibody; NOGOA antibody; NOGOC antibody; NSP antibody; NSP CL antibody; reticulon 4 antibody; Reticulon 5 antibody; RTN X antibody; RTN4 antibody; RTN4 A antibody; RTN4 B1 antibody; RTN4 B2 antibody; RTN4 C antibody; SP1507 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RTN4/NOGO recombinant protein (Accession Number: NM_007008). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114865(RTN4 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:114865(RTN4 antibody) at dilution of 1:100 (under 10x lens)
1X10^6 SH-SY5Y cells were stained with 0.2ug RTN4 antibody (Catalog No:114865, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. RTN4 (also known as Neurite outgrowth inhibitor or Nogo) is a myelin-associated neurite growth inhibitory protein. There are three isoforms: Nogo-A, Nogo-B, and Nogo-C.
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