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Product Name
RtcB-Specific antibody
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Description
RtcB-Specific Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells, HepG2 cells, mouse brain tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 55kd
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
C22orf28 antibody; FAAP antibody; HSPC117 antibody; RtcB antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RtcB-Specific recombinant protein (Accession Number: NM_014306). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114855(C22orf28 antibody) at dilution of 1:500
IP Result of anti-C22orf28 (IP:Catalog No:114855, 3ug; Detection:Catalog No:114855 1:500) with HeLa cells lysate 3000ug.
Immunofluorescent analysis of HeLa cells using Catalog No:114855(C22orf28 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
RTCB (also known as HSPC117, C22orf28, FAAP and D10Wsu52e) is an essential subunit of a tRNA ligase complex that is involved in RNA repair and stress-induced splicing. As a multifunctional protein, RTCB is also a cell adhesion protein and is important in embryo and placenta development. Recently RTCB has been identified as UPR RNA ligase catalyzing unconventional XBP1 mRNA splicing. This antibody specifically recognizes endogenous RTCB protein. (25087875)
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References
- Lu Y, Liang FX, Wang X. A synthetic biology approach identifies the mammalian UPR RNA ligase RtcB. Molecular cell. 55(5):758-70. 2014.
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