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Product Name
RRAS2 antibody
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Description
RRAS2 Rabbit Polyclonal antibody. Positive IF detected in A431 cells. Positive IHC detected in human lung cancer tissue, human heart tissue. Positive IP detected in NIH/3T3 cells. Positive WB detected in NIH/3T3 cells, mouse pancreas tissue. Observed molecular weight by Western-blot: 23 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Ras like protein TC21 antibody; Ras related protein R Ras2 antibody; RRAS2 antibody; TC21 antibody; TeratOCarcinoma oncogene antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RRAS2 recombinant protein (Accession Number: NM_012250). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:114921(RRAS2 antibody) at dilution of 1:800
IP Result of anti-RRAS2 (IP:Catalog No:114921, 4ug; Detection:Catalog No:114921 1:800) with NIH/3T3 cells lysate 4000ug.
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:114921(RRAS2 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:114921(RRAS2 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of A431 cells using Catalog No:114921(RRAS2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
RRAS2 represents related RAS viral (r-ras) oncogene homolog 2
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References
- Dong G, Guo Y, Cao H. Long-term effects of repeated superovulation on ovarian structure and function in rhesus monkeys. Fertility and sterility. 102(5):1452-1457.e1. 2014.
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