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RPS3 antibody

Cat.#: 114843

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Product Information

  • Product Name
    RPS3 antibody
  • Documents
  • Description
    RPS3 Rabbit Polyclonal antibody. Positive IP detected in mouse testis tissue. Positive WB detected in mouse testis tissue, Neuro-2a cells, PC-12 cells. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 26.7 kDa
  • Tested applications
    ELISA, WB, IHC, IP
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    40S ribosomal protein S3 antibody; FLJ26283 antibody; FLJ27450 antibody; ribosomal protein S3 antibody; RPS3 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of RPS3 recombinant protein (Accession Number: NM_001005). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:114843(RPS3 antibody) at dilution of 1:500

    mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:114843(RPS3 antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:114843(RPS3 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:114843(RPS3 antibody) at dilution of 1:50 (under 10x lens)

    IP Result of anti-RPS3 (IP:Catalog No:114843, 3ug; Detection:Catalog No:114843 1:600) with mouse testis tissue lysate 4000ug.

    IP Result of anti-RPS3 (IP:Catalog No:114843, 3ug; Detection:Catalog No:114843 1:600) with mouse testis tissue lysate 4000ug.

  • Background
    40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival.
  • References
    • Hegde V, Yadavilli S, Deutsch WA. Knockdown of ribosomal protein S3 protects human cells from genotoxic stress. DNA repair. 6(1):94-9. 2007.
    • Hegde V, Yadavilli S, McLaughlin LD, Deutsch WA. DNA repair efficiency in transgenic mice over expressing ribosomal protein S3. Mutation research. 666(1-2):16-22. 2009.
    • Yadavilli S, Mayo LD, Higgins M, Lain S, Hegde V, Deutsch WA. Ribosomal protein S3: A multi-functional protein that interacts with both p53 and MDM2 through its KH domain. DNA repair. 8(10):1215-24. 2009.
    • Ghosh S, Kaw M, Patel PR. Mice with null mutation of Ceacam I develop nonalcoholic steatohepatitis. Hepatic medicine : evidence and research. 2010(2):69-78. 2010.
    • Pham TH, Gao X, Tsai K, Olsen R, Wan F, Hardwidge PR. Functional differences and interactions between the Escherichia coli type III secretion system effectors NleH1 and NleH2. Infection and immunity. 80(6):2133-40. 2012.
    • Pham TH, Gao X, Singh G, Hardwidge PR. Escherichia coli virulence protein NleH1 interaction with the v-Crk sarcoma virus CT10 oncogene-like protein (CRKL) governs NleH1 inhibition of the ribosomal protein S3 (RPS3)/nuclear factor κB (NF-κB) pathway. The Journal of biological chemistry. 288(48):34567-74. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"