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Product Name
RPS27 antibody
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Description
RPS27 Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive IHC detected in human endometrial cancer tissue. Positive WB detected in MCF7 cells, SGC-7901 cells. Positive IP detected in SGC-7901 cells. Observed molecular weight by Western-blot: 9 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
40S ribosomal protein S27 antibody; Metallopan stimulin 1 antibody; MPS 1 antibody; MPS1 antibody; ribosomal protein S27 antibody; RPS27 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RPS27 recombinant protein (Accession Number: NM_001030). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:114838(RPS27 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:114838(RPS27 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:114838(RPS27 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using RPS27 antibody Catalog No:114838 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-RPS27 (IP:Catalog No:114838, 4ug; Detection:Catalog No:114838 1:500) with SGC-7901 cells lysate 2400ug.
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Background
RPS27 represents ribosomal protein S27
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