-
Product Name
RPS26 antibody
- Documents
-
Description
RPS26 Rabbit Polyclonal antibody. Positive IP detected in mouse lung tissue. Positive WB detected in mouse ovary tissue, mouse lung tissue. Positive IHC detected in human breast cancer tissue, human kidney tissue. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 18-21kd
-
Tested applications
ELISA, WB, IF, IHC, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
40S ribosomal protein S26 antibody; ribosomal protein S26 antibody; RPS26 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of RPS26 recombinant protein (Accession Number: NM_001029). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
-
Validations
mouse ovary tissue were subjected to SDS PAGE followed by western blot with Catalog No:114837(RPS26 antibody) at dilution of 1:200
Immunofluorescent analysis of MCF-7 cells, using RPS26 antibody Catalog No:114837 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-RPS26 (IP:Catalog No:114837, 4ug; Detection:Catalog No:114837 1:500) with mouse lung tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:114837(RPS26 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:114837(RPS26 Antibody) at dilution of 1:50 (under 40x lens)
-
Background
Diamond-Blackfan anemia (DBA) is a congenital red cell aplasia that is usually diagnosed during early infancy, and strikingly, in addition to defects in red cell maturation the disorder is associated with various physical anomalies in about 40% of patients. To date, heterozygous mutations in nine RP genes have been conclusively associated with DBA, and RPS26 is one of them. There are nine mutation found in RPS26 from DBA patients.
-
References
- Cui D, Li L, Lou H. The ribosomal protein S26 regulates p53 activity in response to DNA damage. Oncogene. 33(17):2225-35. 2014.
- Li Q, Makri A, Lu Y. Genome-wide search for exonic variants affecting translational efficiency. Nature communications. 4:2260. 2013.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"