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Product Name
RPS16 antibody
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Description
RPS16 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer. Positive IF detected in MCF-7. Positive WB detected in HeLa cells, Jurkat cells. Observed molecular weight by Western-blot: 16kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
RPS16 antibody; ribosomal protein S16 antibody; 40S ribosomal protein S16 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RPS16 recombinant protein (Accession Number: NM_001020). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114831(RPS16 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:114831(RPS16 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:114831(RPS16 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using RPS16 antibody Catalog No:114831 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
RPS16, the 40S ribosmal protein S16, belongs to the ribosomal protein S9P family. It is located in the cytoplasm and can be acetylated. RPS16 is extensively involved in rRNA processing, translational elongation, initiation and termination via its RNA binding activity. Catalog#15603-1-AP is a rabbit polyclonal antibody raised against full-length RPS16 of human origin.
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References
- Huang R, Yu M, Li CY. New insights into the functions and localization of nuclear CCT protein complex in K562 leukemia cells. Proteomics. Clinical applications. 6(9-10):467-75. 2012.
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