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Product Name
RPS14 antibody
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Description
RPS14 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells, U2OS cells. Positive IHC detected in human liver tissue. Positive WB detected in HepG2 cells, HeLa cells, mouse liver tissue. Observed molecular weight by Western-blot: 16kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
40S ribosomal protein S14 antibody; EMTB antibody; ribosomal protein S14 antibody; RPS14 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RPS14 recombinant protein (Accession Number: NM_005617). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:114829(RPS14 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver using Catalog No:114829(RPS14 antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:114829(RPS14 antibody) at dilution of 1:200 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using RPS14 antibody Catalog No:114829 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
The mammalian ribosome consists of 4 RNA species and approximately 80 different proteins. The ribosomal proteins are encoded by complex gene families that include at least 1 active intron-containing gene and multiple processed pseudogenes[ PMID 3785212]. The RPS14 gene, encoding a ribosomal protein that belongs to the 40S ribosome, and mapping to the commonly deleted region (CDR) of the 5q- syndrome, shows haploinsufficiency in the CD34+ cells of patients with this disorder[PMID:22430631].
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References
- Fujimura K, Sasaki AT, Anderson P. Selenite targets eIF4E-binding protein-1 to inhibit translation initiation and induce the assembly of non-canonical stress granules. Nucleic acids research. 40(16):8099-110. 2012.
- Iida R, Ueki M, Yasuda T. Identification of interacting partners of Human Mpv17-like protein with a mitigating effect of mitochondrial dysfunction through mtDNA damage. Free radical biology & medicine. 87:336-45. 2015.
- Rinker EB, Dueber JC, Qualtieri J. Differential expression of ribosomal proteins in myelodysplastic syndromes. Journal of clinical pathology. 69(2):176-80. 2016.
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